Stem Cell Research
The stem cell research team at CReATe studies how specialized cells isolated from the umbilical cord can help repair tissues and organs impacted by disease or injury. Human umbilical cord perivascular cells (HUCPVCs) are a type of adult stem cell also known as “mesenchymal stem cells or MSCs”. MSCs isolated from various parts of the body have gained a lot of attention for their utility in regenerative medicine because they can act like “multi-target” drugs. Our studies to date suggest that HUCPVCs are a young source of stem cells with greater regenerative capacity than older sources of MSCs, such as those derived from the bone marrow.
Our studies aim to answer the following questions:
- Do HUCPVCs help regenerate specific tissues and organs? (see 3 active areas of research below)
- How do HUCPVCs work to repair damaged or injured tissues and organs?
- How can we grow or prepare HUCPVCs to maximise their regenerative properties and develop new cell therapies?
- Bazzigaluppi P, Beckett TL, Koletar MM, Hill ME, Lai A, Trivedi A, Thomason L, Dorr A, Gallagher D, Librach CL, McLaurin J, Stefanovic B. Combinatorial treatment using umbilical cord perivascular cells and Aβ clearance rescues vascular function following transient hypertension in a rat model of Alzheimer's Disease. Hypertension. 2019 Oct;74(4):1041-1051.
- Gauthier-Fisher A, Kauffman A, Librach CL. Potential Use of Stem Cells in Fertility Preservation. Andrology. 2019 Sept. (Invited review).
- Gauthier-Fisher A, Szaraz P, Librach CL. Pericytes in the Umbilical Cord. Part of “Biology of Pericytes: Development, Homeostasis and Disease”, Springer Nature series “Advances in Experimental Medicine and Biology (ISSN:0065-2598)”. 2019;1122:211-233.
- Szaraz P, Librach M, Mander P, Hoseini B, Librach M, Iqbal F, Librach CL. A solution to prevent secondary flow in adherent cell cultures. Biology Open. 2019 Jul 25;8(7). pii: bio045294.
- Vawda R, Badner A, Hong J, Mikhail M, Lakhani A, Dragas R, Xhima K, Barretto T, Librach CL, Fehlings MG. Early intravenous infusion of mesenchymal stromal cells exerts an age-dependent beneficial effect on neurovascular integrity and neurobehavioural recovery after traumatic cervical spinal cord injury: Evidence of superiority of first trimester umbilical cord perivascular cells. STEM CELLS Translational Medicine. 2019 Jul;8(7):639-649.
- Gat I, Maghen L, Filice M, Kenigsberg S, Wyse B, Zohni K, Szaraz P, Gauthier Fisher A, Librach CL. Initial germ cell to somatic cell ratios impacts the efficiency of SSC expansion in vitro. Systems Biology in Reproductive Medicine. 2018 Feb;64(1):39-50.
- Iqbal F, Szaraz P, Librach Matthew, Gauthier-Fisher A, Librach CL. Angiogenic potency evaluation of cell therapy candidates by a novel application of the in vitro aortic ring assay. Stem Cell Research and Therapy. 2017 Aug 14;8(1):184.
- Szaraz P, Yarden G, Iqbal F, Librach CL. In vitro differentiation of human mesenchymal stem cells into functional cardiomyocyte-like cells. Journal of Visualized Experiments. 2017 Aug 9;(126), e55757.
- Gat I, Maghen L, Felice M, Wyse B, Zohni K, Jarvi K, Lo K, Gauthier-Fisher A, Librach CL. Optimal culture conditions are critical for efficient expansion of human testicular somatic and germ cells in vitro. Fertility and Sterility. 2017 Mar;107(3):595-605.e7
- Shlush E, Maghen L, Swanson S, Kenigsberg S, Moskovtsev S, Barretto T, Gauthier-Fisher A, Librach CL. In vitro generation of Sertoli-like and haploid spermatid-like cells from human umbilical cord perivascular cells. Stem Cell Research and Therapy. 2017 Feb 15;8(1):37.
- Iqbal F, Szaraz P, Librach M, Gauthier-Fisher A, Li R, Librach CL. The aortic ring co-culture assay: a convenient tool to assess the angiogenic potential of mesenchymal stromal cells (MSCs) in vitro. Journal of Visualized Experiments: JoVE 2017; (127): 56083
- Maghen L, Shlush E, Gat I, Felice M, Barretto T, Jarvi K, Lo K, Gauthier-Fisher A, Librach CL. Human umbilical perivascular cells (HUCPVC’s): a novel source of mesenchymal stromal-like cells MSCs to support the regeneration of the testicular niche. Reproduction. 2017 Jan; 153:85-95.
- Szaraz P, Iqbal F, Librach M, Maghen L, Barretto T, Kenigsberg S, Gauthier-Fisher A, Librach CL. In vitro differentiation of first trimester human umbilical cord perivascular cells into contracting cardiomyocyte-like cells. Stem Cells International. 2016: Article ID 7513252.
- Hong SH, Maghen L, Kenigsberg S, Teichert A, Rameloo A, Shlush K, Szaraz P, Pereira S, Lulat A, Xiao R, Yie S, Gauthier-Fisher A, Librach CL. Ontogeny of human umbilical cord perivascular cells: molecular and fate potential changes during gestation. Stem Cells and Development. 2013 Sep 1;22(17):2425-2439
- Barretto T, Park E, Telliyan T, Liu E, Gallagher D, Librach CL, Baker A. Vascular dysfunction after modelled TBI is rescued with administration of umbilical cord derived mesenchymal stromal cells and is associated with modulation of the angiogenic response. Journal of Neurotrauma. 2021 Jun 9. doi: 10.1089/neu.2021.0158
- Mashiach J, Zohni K, Lopez L, Filice M, Garcia M, Wyse B, Glass K, Dviri M, Baram S, Gauthier-Fisher A, Librach CL. Human Umbilical Cord Perivascular Cells (HUCPVC) prevent chemotherapeutic drug-induced male infertility in a mouse model. Fertility and Sterility Science. 2021 Feb;2(1):24-32.
- Zohni K, Lopez L, Szaraz P, Filice M, Wyse BA, Garcia M, Gat I, Glass K, Gauthier-Fisher A, Librach CL. Human umbilical cord perivascular cells maintain regenerative traits following exposure to cyclophosphamide. Cancer Letters. 2020 Dec 30;501:133-146.
- Szaraz P, Mander P, Gasner N, Librach M, Iqbal F, Librach CL. Glucose withdrawal induces Endothelin 1 release with significant angiogenic effect from first trimester (FTM), but not in term human umbilical cord perivascular cells (HUCPVC). Angiogenesis. 2020 May;23(2):131-144.
- Barretto T, Park K, Maghen L, Park E, Kenigsberg S, Gallagher D, Gauthier-Fisher A, Librach CL, Baker A. Axon degeneration is rescued with human umbilical cord perivascular cells – a potential candidate for neuroprotection after traumatic brain injury. Stem Cells Dev. 2020 Feb 15;29(4):198-211.
- Gallagher D, Siddiqui F, Charlat M, Chaudry E, Moolla S, Fish J, Gauthier-Fisher A, Librach CL. Mesenchymal Stromal Cells Modulate Peripheral Stress-Induced Innate Immune Activation Indirectly Limiting Emergence of Neuroinflammation-Drive Depressive and Anxiety-like Behaviors. Biological Psychiatry. 2019 Nov 1; 86:712-724.